Diagnosis of Sexually Transmitted Diseases: Methods and Protocols/ Editors: Colin R. Mackenzie and Birgit Henrich

Includes Bibliographies and Index

1. Overview of Molecular Biological Methods for the Detection of Pathogens Causing Sexually Transmitted Infections Fernando Vazquez, Santido Melon, Luis Otero, and Maria de Ona
2. Guidelines for the Qualitative Detection of Viral Genomes in Dried Blood Spots Davide Gibellini, Elisa De Crignis, and Maria Carla Re
3. Guidelines for the Quantification of HIV and HCV in Small Volume Whole Blood Samples Tony Bruns and Katrin Steinmetzer
4. Guidelines for High-Resolution Genotyping of Chlamydia trachomatis Using Multilocus Sequence Analysis Linus Christerson and Bjoern Herrmann
5. Guidelines for the Use of Molecular Tests for the Detection and Genotyping of Human Papilloma Virus from Clinical Specimens Sin Hang Lee
6. The Molecular Diagnosis of Sexually Transmitted Genital Ulcer Disease Cheng-Yen Chen and Ronald C. Ballard
7. Validation of a Sensitive and Specific Real-Time PCR for Detection and Quantitation of Hepatitis B Virus Covalently Closed Circular DNA in Plasma of Chronic Hepatitis B Patients R. B. Takkenberg, S. Menting, and M.G.H.M. Beld
8. Protocol for the Detection of Mycoplasma genitalium by PCR from Clinical Specimens and Subsequent Detection of Macrolide Resistance Mediating Mutations in Region V of the 23S rRNA Gene Jorgen Skov Jensen
9. Protocols for Detection and Typing of Treponema pallidum Using PCR Methods S.M. Bruisten
10. Protocol for the Extraction of DNA from Dried Blood in the Diagnosis of Convenital CMV Infection Jutte J.C. de Vries, Maria Barbi, Sandro Binda, and Eric C.J. Claas
11. Protocol for the Use of PCR-Denaturing Gradient Gel Electrophoresis and Quantitative PCR to Determine Vaginal Microflora Constitution and Pathogens in Bacterial Vaginosis Beatrice Vitali, Elena Biagi, and Patrizia Brigidi
12. Protocol for the Use of a Bead Array for the Multiple Detection of Genotype of Chlamydia trachomatis Chung-Ter uhang and Shu-Ying Li
13. Protocol for the Detection and Genotyping of Human Papilloma viruses Using a Liquid Bead Microarray Assay (LBMA) Stephen Cherne and Qinghua Feng
14. Protocol for the Use of Enzyme-Linked Hybridisation Assays for Genital Ulcer Disease Seweryn Bialasiewicz, Ian M. Mackay, David M. Whiley, and Theo P. Sloots
15. Protocol for the Rapid Detection of the Urogential Tract Mollicutes and Chlamydia with Concomitant LGV -(sub)typing Nadine Moebius, Wibke Brenneisen, Anke Schaeffer, and Birgit Henrich
16. Protocol for the Use of Self-Reporting Duplex Mutation Primers to Detect PCR Products in the Diagnosis of HBV Qian-Feng Xia 17. Protocol for the Use of a Rapid Real-Time PCR Method for the Detection of HIV-1 Proviral DNA Using Double-Stranded Primer Chou-Pong Pau, Susan K. Wells, and Timothy C. Granade
18. Protocol for the Use of Light Upon Extension (LUX) Real-Time PCR for the Determination of Viral Load in HBV Infection Guimin Li, Wangfeng Li, and Lixia Liu
19. Protocol for the Use of a Silica Nanoparticle-Enhanced Microcantilever Sensor-Based Method to Detect HBV at Femtomolar Concentrations Sang-Myung Lee, Kyo Seon Hwang, Sang Kyung Kim, and Tae Song Kim
20. Protocol for the Detection of Treponema pallidum in Paraffin-Embedded SpecimensCheng-Yen Chen and Allan Pillay
21. Guidelines for the Use of Molecular Biological Methods to Detect Sexually Transmitted Pathogens in Cases of Suspected Sexual Abuse in Children Margaret R. Hammerschlag and Charlotte A. Gaydos
22. Protocol for the Molecular Detection of Antibiotic Resistance Mechanisms in Neisseria gonorrhoeae Namraj Goire, Theo P. Sloots, Michael D. Nissen, and David M. Whiley
23. Protocol for a Facile Multiplex PCR for Multi-Antimicrobial Resistance and Gonococcus Detection Ratana Lawung, Rungrot Cherdtrakulkiat, and Virapong Prachayasittikul
24. Protocol for Gene Expression Profiling Using DNA Microarrays in Neisseria gonorrhoeae Lydgia A. Jackson and David W. Dyer 25. Protocol for the Detection of Human Leukocyte Antigen Variants and Sexually Transmitted Infections: From Genotyping to Immunoassays Jianming Tang and Anju Bansal
26. Protocol for the Clonal Analysis of NK Cell Effector Functions by Multi-Parameter Flow Cytometry Kathrin Schoenberg, Maryam Hejazi, and Markus Uhrberg
27. Protocol for a Mammalian Cell-Based Assay for Monitoring the HIV-1 Protease Activity Chitra Rajakuberan, Brett J. Hilton, and Roland Wolkowicz
28. Self Collection of Specimens for Nucleic Acid-Based Diagnosis of Pharyngeal, Cevicovaginal, Urethral and Rectal Neisseria gonorrhoeae and Chlamydia trachomatis Infections Vivian Levy, Craig S. Blackmore, and Jeffrey D. Klausner
29. Sexually Transmitted Diseases - Reflections on Metaphors and Ethics Gisela Badura-Lotter